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Part:BBa_K2609026:Design

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-26)
Revision as of 19:18, 5 October 2018 by Dharanish (Talk | contribs) (→‎Source)


Lambda Red Recombinases under T7 expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1460
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is under a T7 adapter having T7 promoter upstream and a T7 terminator downstream. It has HindIII and NheI sites on either sides which was used for easy cloning into the adapter.

Source

Assembled using part <a href="https://parts.igem.org/Part:BBa_K1433005"> BBa_K14433005 </a> and a T7 adapter.

References