Generator
Part:BBa_K2609026:Design
Designed by: Bhaskar Kumawat Group: iGEM18_IISc-Bangalore (2018-09-26)
Lambda Red Recombinases under T7 expression system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1943
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1460
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is under a T7 adapter having T7 promoter upstream and a T7 terminator downstream. It has HindIII and NheI sites on either sides which was used for easy cloning into the adapter.
Source
Assembled using part BBa_K1433005 and a T7 adapter.