Regulatory
p(osmY)

Part:BBa_J45993:Design

Designed by: Stephen Payne   Group: iGEM06_MIT   (2006-07-11)
Revision as of 19:57, 4 February 2008 by Stephen (Talk | contribs) (→‎Design Notes)


Minimal stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Forward Primer

5'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3'

Reverse Primer

5'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3'

Source

This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.

References

<biblio>

  1. Yim pmid=8282684
  2. Schellhorn pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.