Reporter

Part:BBa_K2810001

Designed by: Geraint Parry   Group: iGEM18_Cardiff_Wales   (2018-08-24)
Revision as of 17:31, 24 August 2018 by Registry (Talk | contribs)

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GUS reporter for plant expression

The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry.

The GUS gene is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This has been used to generate the composite reporter constructs detailed in the 2018 Cardiff_Wales Parts page


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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