Composite
Part:BBa_K2442406
Designed by: Ruiyang He, James Provan Group: iGEM17_Glasgow (2017-09-07)
[split GFP module 2] lacI regulated promoter + RBS + GFP C-term + double terminator
This is a composite of the GFP C-term split subunit (GFP2) K2442400, the well characterised double-terminator construct B0015, and the well characterised lacI regulated promoter R0011
Usage and Biology
this composite was used for assembly of larger composite split-GFP AND-gate constructs.
- K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 264
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Categories
Parameters
None |