Translational_Unit

Part:BBa_K2213009

Designed by: Adam Hannaford   Group: iGEM17_Manchester   (2017-10-09)
Revision as of 02:46, 2 November 2017 by Trglenaldo (Talk | contribs)


LowPromoter_PduD(1-20)_mCherry_cgPPK2
The PduD(1-20) localisation tag, mCherry and PPK (from C. glutamicum) under control of the low strength Anderson promoter (https://parts.igem.org/Part:BBa_J23105). PPK is tagged with His6 (see figure 1).

Low-tag-mcherry-PPK.png
Figure 1: Schematic of LowPromoter_PduD(1-20)_mCherry_cgPPK2

The Manchester iGEM team has combined this part with BBa_K2213002, which contains EutLK under the control of an araBad promoter, to make the composite part, BBa_K2213013. The composite part showed that cgPPk2 was localised in the Eut microcompartment. Characterisation of BBa_K2213013 can be found at: https://parts.igem.org/Part:BBa_K2213013 and characterisation of BBa_K2213002 can be found at: https://parts.igem.org/Part:BBa_K2213002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 908
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 146
    Illegal SapI.rc site found at 1587


[edit]
Categories
Parameters
None