Part:BBa_T2028

Chitin binding internal domain

This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins.

Usage and Biology

The chitin binding domain tag is used for protein purification using a chitin column. CBD can be placed on either the N- or C-terminus of proteins and is usually used in conjunction with a self-splicing intein tag. Purification of a CBD-tagged protein is a one-step purification process that yields very pure protein with low miligram yields. The primary disadvantage is that the purification must be done in the absence of thiol-containing reducing agents until the elution step. However, the matrix is compatible with high salt and nonionic detergents.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]