Part:BBa_K2440020

miR-184 target sequence

It is the target sequence of miR-184, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-184 in an Ago2 dependent manner, that is, knockdown of miR-184 was achieved by transfecting cells with miRNA locker.

Hsa-miR-184 was firstly found in mouse eyes. And it is aslo identified by BLAST searching of a cloned miRNA in another species (Homo sapiens; Mus musculus; Fugu rubripes ; Danio rerio; Drosophila melanogaster)1.

In vitro functional studies demonstrated that miR-184 significantly inhibited colorectal cancer cell proliferation, migration and invasion. Notably, insulin-like growth factor 1 receptor (IGF-1R) was identified as a direct target of miR-184 in colorectal cancer. Furthermore, the functions of IGF-1R small interfering RNA were similar to those induced by miR-184 in colorectal cancer, suggesting IGF-1R as a functional target of miR-184 in colorectal cancer. So miR-184 may be a novel therapeutic strategy regimen of targeted therapy for colorectal cancer2.

Moreover, miR-184 can inhibit proliferation and promotes apoptosis of human colon cancer SW480 and HCT116 cells by downregulating C-MYC and BCL-23.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.