Part:BBa_K2440020
miR-184 target sequence
It is the target sequence of miR-184, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-184 in an Ago2 dependent manner, that is, knockdown of miR-184 was achieved by transfecting cells with miRNA locker.
Hsa-miR-184 was firstly found in mouse eyes. And it is aslo identified by BLAST searching of a cloned miRNA in another species (Homo sapiens; Mus musculus; Fugu rubripes ; Danio rerio; Drosophila melanogaster).1
In vitro functional studies demonstrated that miR-184 significantly inhibited colorectal cancer cell proliferation, migration and invasion. Notably, insulin-like growth factor 1 receptor (IGF-1R) was identified as a direct target of miR-184 in colorectal cancer. Furthermore, the functions of IGF-1R small interfering RNA were similar to those induced by miR-184 in colorectal cancer, suggesting IGF-1R as a functional target of miR-184 in colorectal cancer. So miR-184 may be a novel therapeutic strategy regimen of targeted therapy for colorectal cancer.2
Moreover, miR-184 can inhibit proliferation and promotes apoptosis of human colon cancer SW480 and HCT116 cells by downregulating C-MYC and BCL-2.3
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2.MicroRNA-184 inhibits cell proliferation and metastasis in human colorectal cancer by directly targeting IGF-1R. Wu G, Liu J, Wu Z, Wu X, Yao X.
3.MicroRNA-184 inhibits proliferation and promotes apoptosis of human colon cancer SW480 and HCT116 cells by downregulating C-MYC and BCL-2. Wang YB, Zhao XH, Li G, Zheng JH, Qiu W.
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