Chassis/Cell-Free Systems/Commercial E.coli S30/Expression Lifespan

Revision as of 16:54, 21 October 2007 by Cheuk Ka Tong (Talk | contribs) (→‎Expression Lifespan)

Commercial E. coli S30

Cell-Free Systems Chassis description Optimum temperature Peak time Product stability Expression capacity Expression lifespan

Expression Lifespan

Graph 2. Graph of rate of GFP synthesis (mol per min) against time (minutes) at 4oC (dark blue), 15oC (pink), 20oC (yellow) and 30oC (light blue). The simple constitutive gene expression device BBa_I13522 has been used. Each of the four coloured lines show average measurements based on three replicates of the experiment. This graph is derived from Graph 1, with addition data from our GFP calibration curve. Please visit our [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.2 OpenWetWare] for the detailed protocol and raw data of the experiment.