Part:BBa_K2333437
Copper sensor with Protein Degradation Tag A
This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of protein degradation tagged RFP from the downstream PcopA promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Characterized by UMaryland 2017, fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner.
Usage and Biology
This part contains CueR repressor under the constitutive promoter BBa_J23102 combined with pdt-A tagged RFP under the control of the PcopA promoter. Protein degradation tag A is the strongest of the 6 protein degradation tags that William and Mary 2017 characterized, and is associated with the E. Coli orthogonal protease mf-Lon (BBa_K2333011). This part is contained in a suite of parts that allowed William and Mary 2017 to demonstrate persistence of speed-change effects for protein outputs beyond simple reporter proteins, and provides an example of a practical application of their speed-control system to improve biosensor output speed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1229
Illegal AgeI site found at 1341 - 1000COMPATIBLE WITH RFC[1000]
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