Part:BBa_K2333011

mf-Lon

This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1834
  Illegal AgeI site found at 1918
  Illegal AgeI site found at 2124
  Illegal AgeI site found at 2149
  
• 1000
  COMPATIBLE WITH RFC[1000]

Modifications in Sequence : iGEM21_IISER-Tirupati_India

BBa_K3889020 is a modified version specific to our project's assembly. Mutated multiple RE sites to make it assembly compatible.