Tag

Part:BBa_K2333003

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-06-14)
Revision as of 15:14, 29 October 2017 by Cemonette (Talk | contribs)


mf-Lon Protein Degradation Tag C (medium-strong)

This protein degradation tag (pdt) is degraded by the Lon protease from Mycoplasma florum (mf-Lon) (BBa_K2333011).The Lon protease efficiently recognize and degrade C-terminal ssrA-tagged proteins, functioning in a similar way as ClpXP protease in most gram-negative bacteria. This Lon protease system is orthogonal to E. coli’s endogenous protein degradation machinery. This tag is part of a series of 6 tags (labeled A thru F). pdtA is used as the parental tag to create tags B-F by changing the pdt residues 13–15 for mutagenesis because this region is essential for Lon-mediated degradation. Of this series of tags, this tag is moderately strong, and has high degradation rate. BBa_K2333001-Bba_K2333006 comprise this tag series. See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design via mutagenesis, and for their characterization of degradation rates. Note: pdt B varies from pdt A in that residues 13-15 change from “PTF” to “RAI”.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None