Coding
mRFP1

Part:BBa_E2050

Designed by: ryu   Group: Antiquity   (2005-01-24)
Revision as of 13:43, 29 October 2010 by YHR (Talk | contribs) (Usage and Biology)

derivative of mRFP1, yeast-optimized

mRFP derivative. Ex548nm/Em562. yeast codon optimized.


Usage and Biology

mOrange, a mRFP (DsRed) derivative. Higher quantum yield but blue shifted and slower maturing compared to current (1/05) best red, mCherry.


We used the part BBa_E2050to construct mRFP-fused protein. Sequencing result revealed this part is working.


Tianjin IGEM Team

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//chassis/eukaryote/yeast
//function/reporter/fluorescence
Parameters
abs
colorOrange
emission
emit562
excitation
excite548
lum
proteinmOrange
tagNone