Coding
mRFP1
Part:BBa_E2050
Designed by: ryu Group: Antiquity (2005-01-24)
derivative of mRFP1, yeast-optimized
mRFP derivative. Ex548nm/Em562. yeast codon optimized.
Usage and Biology
mOrange, a mRFP (DsRed) derivative. Higher quantum yield but blue shifted and slower maturing compared to current (1/05) best red, mCherry.
We used the part BBa_E2050to construct mRFP-fused protein. Sequencing result revealed this part is working.
Tianjin IGEM Team
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 763
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/rfp
//chassis/eukaryote/yeast
//function/reporter/fluorescence
//chassis/eukaryote/yeast
//function/reporter/fluorescence
abs | |
color | Orange |
emission | |
emit | 562 |
excitation | |
excite | 548 |
lum | |
protein | mOrange |
tag | None |