Cell-free chassis/Commercial E. coli S30
Revision as of 12:34, 21 October 2007 by Cheuk Ka Tong (Talk | contribs) (→Commercial ''E. coli'' S30)
Commercial E. coli S30
| Cell-Free Systems | Chassis description | Optimum temperature | Peak time | Product stability | Expression capacity | Expression lifespan |
Description
The Commercial E. coli S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). E. coli strain B deficient in OmpT endoproteinase and lon protease activity was used.
The simple constitutive gene expression device BBa_I13522 has been used to characterize this cell-free chassis
References
Please refer to the Promega cell extract user guide for detailed information about the Commercial E. coli S30 extract.