Coding
Part:BBa_K2332020
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-18)
LuxCDABE (E. coli bioluminescence)
Our LIT Bulb will be composed of light sensitive E. coli that can detect sunlight (blue light) to repress bioluminescence (LuxCDABE). In the presence of light transcription of the LuxCDABE for the production of Luciferase and substrates will be inhibited. In the dark, the inhibition will be released and bioluminescence will be induced. This part encodes the necessary requirements for bioluminescence production, codon optimized for E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2562
Illegal AgeI site found at 2829
Illegal AgeI site found at 3357
Illegal AgeI site found at 3938
Illegal AgeI site found at 4385
Illegal AgeI site found at 5263
Illegal AgeI site found at 5478 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |