Translational_Unit

Part:BBa_K2278023:Design

Designed by: Paul ZANONI   Group: iGEM17_INSA-UPS_France   (2017-10-08)
Revision as of 13:24, 9 October 2017 by Pzanoni (Talk | contribs) (→‎Design Notes)


cOT2 antimicrobial peptide with Alpha-Factor Secretion Signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part:BBa_K431009 : glyceraldehyde 3-phosphate dehydrogenase promoter P(GAP)

Part:BBa_K1800001: Alpha-Factor Secretion Signal

Source

The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the proposed by Prajanban et al., 2017. They had determinated the amino acid sequence by mass spectrometry analysis.

Prajanban, B., Jangpromma, N., Araki, T. and Klaynongsruang, S. (2017). Antimicrobial effects of novel peptides cOT2 and sOT2 derived from Crocodylus siamensis and Pelodiscus sinensis ovotransferrins. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1859(5), pp.860-869.

Brogden, K. (2005). Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?. Nature Reviews Microbiology, 3(3), pp.238-250.

References