Part:BBa_K2278023:Design

cOT2 antimicrobial peptide with Alpha-Factor Secretion Signal

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 244
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

A pAOX1 promoter is present on the pPICZalpha vector before the construction. It makes genome recombination easier in Pichia pastoris genome. Part:BBa_K1800001: Alpha-Factor Secretion Signal

Source

The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the proposed by Prajanban et al., 2017. They had determinated the amino acid sequence by mass spectrometry analysis.

References

Prajanban, B., Jangpromma, N., Araki, T. and Klaynongsruang, S. (2017). Antimicrobial effects of novel peptides cOT2 and sOT2 derived from Crocodylus siamensis and Pelodiscus sinensis ovotransferrins. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1859(5), pp.860-869. <p>Brogden, K. (2005). Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?. Nature Reviews Microbiology, 3(3), pp.238-250.