Regulatory
PDF1.2 (sh
Part:BBa_K2404002:Design
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-09-22)
PDF1.2 shortened - a jasmonic acid-induced promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 408
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 517
Design Notes
To isolate this promoter we needed to create a gene construct with the shortened PDF1.2 as the promoter element. We used the Golden Gate cloning system to do this, and so needed to isolate upstream restriction enzyme recognition sites (i.e. Type IIS restriction endonucleases) that are suitable for Golden Gate reactions. This consideration was taken into account when designing primers to isolate this part.
Source
This part comes from Arabidopsis thaliana and was isolated using specific primers.