Regulatory
PDF1.2 (sh

Part:BBa_K2404002:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)


PDF1.2 shortened - a jasmonic acid-induced promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 517


Design Notes

- We isolated genomic DNA from wildtype Arabidopsis thaliana

- We designed primers to amplify the PDF1.2 promotor sequence.
iGEM45 PDF1pF tttCGTCTCtCTCAggagactacgcgagaacggttcc
iGEM46 PDF1pR tttCGTCTCtCTCGcattTGGTAGTGGGTTAATCTTC

- We gel extracted the DNA and digested with BsmBI to introduce the plasmid into pSB1C3.

- This plasmid corresponds to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard.


Source

This part comes from Arabidopsis thaliana and was isolated using specific primers.

References