DNA

Part:BBa_K2308006:Design

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-05)
Revision as of 05:53, 5 October 2017 by Registry (Talk | contribs)

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upstream sequence of the gene crtB in genome of Rhodobacter Sphaeroides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 47
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 162


Design Notes

There's a point mutant in the sequence in order to avoid specific endonuclease site.


Source

from the genome of Rhodobacter Sphaeroides

References