Part:BBa_K2308006:Design

upstream sequence of the gene crtB in genome of Rhodobacter Sphaeroides

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 93
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 47
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 162
  

Design Notes

There's a point mutant in the sequence in order to avoid specific restriction site.

Source

from the genome of Rhodobacter Sphaeroides