Composite

Part:BBa_M50013:Design

Designed by: Maddie Hayes-Lattin   Group: Stanford BIOE44 - S11   (2016-10-27)
Revision as of 21:52, 4 December 2016 by Maddiehl (Talk | contribs) (→‎Design Notes)


Arsenic Sensor (arsR operon promoter and arsR gene)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

arsR produces a repressor protein that binds to the arsR promoter. When arsR levels are sufficiently high, transcription is repressed until arsenic binds to these repressors and allows transcription to start. Additional protein-coding genes can be placed after this promoter-repressor unit. These proteins will only be expressed in the presence of arsenic.

Source

This part can be found in E. Coli chromosomal and plasmid DNA. (Escherichia coli K-12 substr. MG1655 arsR. (2016). Ecocyc.org. Retrieved 26 October 2016, from http://ecocyc.org/gene?orgid=ECOLI&id=EG12235#tab=REGULON).

References