Reporter

Part:BBa_K2070007:Design

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)
Revision as of 10:28, 30 October 2016 by Nasu (Talk | contribs) (→‎Source)


Toehold Trigger Inducible GFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 709


Design Notes

There is no TACTAGAG sequence between toehold RBS sequence and GFP sequense.

Source

Assembly.

References