Reporter

Part:BBa_K2070007:Design

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)


Toehold Trigger Inducible GFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 709


Design Notes

There is no TACTAGAG sequence between toehold RBS sequence and GFP sequense.

Source

Assembly.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, and Peng Yin, (2014, 11) Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell 159, 925–939.