Device
Part:BBa_K1886008
Designed by: Lejian Jiang Group: iGEM16_ZJU-China (2016-10-06)
AND GATE-output-1
This is a report system to test the AND GATE made up of SupD tRNA coding gene and T7 polymerase with two amber mutations. T7 promoter is a very specific promoter which is transcribed only by specific T7 RNA polymerase. Following the PT7 promoter is the reporter gene GFP, Only when the two inputs are given, the GFP gene can be expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 693
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Categories
Parameters
None |