Device

Part:BBa_K1886008

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


AND GATE-output-1

This is a report system to test the AND GATE made up of SupD tRNA coding gene and T7 polymerase with two amber mutations. T7 promoter is a very specific promoter which is transcribed only by specific T7 RNA polymerase. Following the PT7 promoter is the reporter gene GFP, Only when the two inputs are given, the GFP gene can be expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Characterization

BACKGROUND

Overview

Registered Parts in our wiki is the subunit of the entire AND Gate system, each of which has no function. So I will introduce our logical part of our project after the combination of all the subunits in the page of part BBa_K1886016.

Results

Gel electrophoretic analysis

Fig.1 Fig.1 Gel electrophoretic analyses of PCR products
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Categories
Parameters
None