Generator

Part:BBa_K1934061:Experience

Designed by: Mathilde Gonthier   Group: iGEM16_INSA-Lyon   (2016-10-13)
Revision as of 13:51, 15 October 2016 by MattBorel (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1934061

This part has been transferred into e. coli NM522 for expression. After 6 hours at 37° IPTG has been added to the final concentration of 1 mM. The expression culture was grown overnight at 30°. We the used instructions provided on the NiNTA columns kit from Quigen to purify the protein.

User Reviews

UNIQ6a386721b185a116-partinfo-00000000-QINU UNIQ6a386721b185a116-partinfo-00000001-QINU