Generator
Part:BBa_K1934061:Experience
Designed by: Mathilde Gonthier Group: iGEM16_INSA-Lyon (2016-10-13)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1934061
This part has been transferred into E. coli NM522 for expression. After 6 hours at 37°C, IPTG was added to a final concentration of 1mM. The expression culture was grown overnight at 30°C. We then used instructions provided on the Ni-NTA columns kit from Qiagen to purify the protein.
User Reviews
UNIQda51abae329877d5-partinfo-00000000-QINU UNIQda51abae329877d5-partinfo-00000001-QINU