Composite
IRES-eGFP

Part:BBa_K1993034

Designed by: Su Xiaojun   Group: iGEM16_SYSU-MEDICINE   (2016-10-13)
Revision as of 09:23, 18 October 2016 by Icyvodka (Talk | contribs)


IRES-eGFP

In order to construct our device easily since we constructed our plasmids by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions. We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. We also added the gene of eGFP(Details can be seen from BBa_K1993017) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None