Part:BBa_K1993034
IRES-eGFP
In order to construct our device easily by Gateway technology, we constructed a composite part expressing IRES and eGFP which is convenient for running Gateway BP and LR reactions. We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translation initiation in an end-independent manner, as part of the greater process of protein synthesis. We also added the gene of eGFP(Details can be seen from BBa_K1993017) following IRES, which is a modified form of GFP frequently used as a reporter of expression. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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