Composite

Part:BBa_K2042015:Design

Designed by: Amir Pandi   Group: iGEM16_Evry   (2016-08-30)
Revision as of 21:30, 12 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Source)


LDH* Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 699
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was introduced in pSEVA424 plasmid in order to increase the lactate fraction in Pseudomonas putida.

Source

The LDH* were synthesized from the genomic sequence of Lactobacillus delbrueckii 11842

References