Composite

Part:BBa_K2042015:Design

Designed by: Amir Pandi   Group: iGEM16_Evry   (2016-08-30)


LDH* Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 699
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A BamHI site was placed between the promoter and the RBS in order allow expression with other promoter This part was introduced in pSEVA424 plasmid in order to increase the lactate fraction in Pseudomonas putida.

Source

The LDH* were synthesized from the genomic sequence of Lactobacillus delbrueckii 11842

References