Composite

Part:BBa_K1897016:Design

Designed by: Ang Shi Hui   Group: iGEM16_NUS_Singapore   (2016-10-10)
Revision as of 19:35, 10 October 2016 by Registry (Talk | contribs)

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LuxR + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 949
    Illegal NheI site found at 972
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
    Illegal AgeI site found at 992
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 740


Design Notes

There is one HA tag available for characterisation of the LuxR protein produced via western blot.


Source

LuxR was obtained from biobrick part BBa_C0062, which was derived from Vibrio fischeri. GFP was obtained from biobrick part BBa_E0040, which was derived from Aequeora victoria.

References