Coding
HYG
Part:BBa_K1884003:Design
Designed by: YongJing Ping Group: iGEM16_SZU-China (2016-10-07)
Hygromycin B
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 455
Illegal NgoMIV site found at 637 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 910
Design Notes
PIF3 functions as a transcriptional activator, and can be functionally and mechanistically separated from its role in repression of PhyB mediated processes.
Source
photochrome interacting factor 3
References
1.Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983), "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli", Antimicrobial Agents and Chemotherapy, 24 (5): 689–95
2.Kauffman, John (2009), "Analytical Strategies for Monitoring Residual Impurities Best methods to monitor product-related impurities throughout the production process.", BioPharm International, 23: 1–3