Coding
HYG

Part:BBa_K1884003:Design

Designed by: YongJing Ping   Group: iGEM16_SZU-China   (2016-10-07)


Hygromycin B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 455
    Illegal NgoMIV site found at 637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 910


Design Notes

Source

we gain this parts from Shenzhen Engineering Laboratory of Marine Algal Biotechnology.

References

[1]Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983), "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli", Antimicrobial Agents and Chemotherapy, 24 (5): 689–95

[2]Kauffman, John (2009), "Analytical Strategies for Monitoring Residual Impurities Best methods to monitor product-related impurities throughout the production process.", BioPharm International, 23: 1–3