Coding

Part:BBa_K1935001:Design

Designed by: Emilie Gounin   Group: iGEM16_Bordeaux   (2016-10-08)
Revision as of 14:00, 13 October 2016 by Jdesca911e (Talk | contribs) (→‎Design Notes)


DSIP - delta sleep inducing peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleic sequence and coding gene of DSIP is unknown. We decide to convert amino-acid sequence into nucleic sequence. After that we used optimisation codon sequence for Caenorhabditis elegans. We decided to add two DSIP sequence with P2A sequence between them. We also added an ATG codon stop in 5'-end.

Source

Delta sleep-inducing peptide (DSIP): a still unresolved riddle". Journal of Neurochemistry Kovalzon VM and Strekalova TV (2006)

Delta-Sleep-Inducing Peptide (DSIP): A Review MARKUS V. GRAF AND ABBA J.KASTIN

References