Composite
Part:BBa_K1897013:Design
Designed by: Ang Shi Hui Group: iGEM16_NUS_Singapore (2016-10-10)
Listeriolysin O
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1482
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 90
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LLO CDS BBa_K1897012 is shifted to after the HA tag.
Source
Listeriolysin O (LLO) is derived from Listeria monocytogenes and is _______.