Composite
Part:BBa_K1783002:Design
Designed by: Chun Mun Loke, Adam Wahab, Kimia Abtahi, Iowis Zhu Group: iGEM15_UMaryland (2015-07-18)
Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 619
Illegal AgeI site found at 731 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. The BioBrick was transformed into both E.coli K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing.
Source
Promoter, RBS, LVA tag from E. coli RFP from Discosoma striata