Reporter

Part:BBa_K1638007

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-10)
Revision as of 12:24, 23 July 2015 by Jpettersen (Talk | contribs) (→‎Fluorescence wavelengths)

GFP reporter with flexible linker

This part codes for a GFP reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression. This part also contains two transcriptional terminators.

Fluorescence wavelengths

  • Excitation max - 501nm
  • Emission max - 511nm

For further description of the GFP read here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674


[edit]
Categories
Parameters
None