Generator
ccdB

Part:BBa_P1016:Design

Designed by: Reshma Shetty   Group: iGEM06_Bangalore   (2007-01-29)
Revision as of 18:45, 29 January 2007 by Rshetty (Talk | contribs)

ccdB cassette without ccdA-


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 323


Design Notes

ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.


This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part (thus the coding region of ccdB is similar to commercially available versions of ccdB). It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.

Source

This part is derived from BBa_P1010 but it lacks ccdA-. It was synthesized via direct synthesis by Codon Devices as a part of a larger part BBa_I51001.

References