DNA

Part:BBa_K1732007

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)
Revision as of 01:07, 17 September 2015 by Donnalee (Talk | contribs)

pSB1C3-J23100-B0034-BFP-His-B0015

pSB1C3-J23100-B0034-BFP-His-B0015

His tagged mTagBFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 607
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 518
    Illegal BsaI.rc site found at 683


[edit]
Categories
Parameters
None