Coding

Part:BBa_K1021001

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-08-30)
Revision as of 03:23, 29 October 2013 by O.Spassibojko (Talk | contribs) (→‎Usage and Biology)

nptII (neomycin phosphotransferase)

The nptII gene encodes neomycin phosphotransferase, which confers resistance to geneticin, kanamycin, and related antibiotics.

Usage and Biology

  • Cornell iGEM demonstrated the functionality of this part via transformation (in vector pNG) into C. heterosporus. The fungal transformants:

PNG.JPG

  • This part has been used in the construction of composite and generator parts BBa_K1021015, BBa_K1021029, BBa_K1021027, and BBa_K1021032.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 478
    Illegal SapI.rc site found at 688


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Categories
Parameters
None