Generator
KanMX(lox)

Part:BBa_K300989:Experience

Designed by: Lorenzo Pasotti, Giacomo Zambianchi, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)
Revision as of 12:00, 24 October 2010 by Lor18 (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K300989

User Reviews

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User Reviews

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UNIPV-Pavia iGEM 2010

This part was successfully used in the design of the BBa_K300001 integrative base vector for S. cerevisiae.

When transformed in S288C S. cerevisiae strain (BBa_K300979), BBa_K300989 actually conferred the resistance to the antibiotic G418 (geneticin) at 200 ug/ml on YPD agar plates, while the "no DNA" negative control did not show colonies. See the BBa_K300001 Experience page for details and pictures.

The loxP sites recombination capability has not been tested yet.

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