Part:BBa_K1499400:Design
Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this gene was obtained from the Polistes dominula genome[1]. We codon-optimized it for expression in E. coli and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.
Note: This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality.
Source
Polistes dominula genome.
References
Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB
Studies of amino acid composition of nest protein in other Polistes species: http://link.springer.com/article/10.1007/PL00001733 http://link.springer.com/article/10.1007/s101640070003 http://link.springer.com/article/10.1007/BF00997166 http://link.springer.com/article/10.1007/BF00982111