Composite

Part:BBa_K1529797:Design

Designed by: Shoko Suzuki   Group: iGEM14_Tokyo_Tech   (2014-10-08)
Revision as of 10:28, 12 October 2014 by Kohdai (Talk | contribs) (→‎References)

Plux-CmR-RhlI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sequence confirmed



Source

Composite of BBa_K395162, BBa_B0034 and BBa_C0070.

BBa_K395162 : Lux-promoter_CmR From Tokyo_Tech 2010

BBa_B0034 : RBS From kit plate

BBa_C0070 : RhlI From kit plate

References

1.Bo Hu et al. (2010) An Environment-Sensitive Synthetic Microbial Ecosystem. PLoS ONE 5(5): e10619
2.Jennifer M. Henke et al. (2004) Bacterial social engagements. TRENDS in Cell Biology 14: 11
3.Gabriella Pessi et al. (2000) Transcriptional Control of the Hydrogen Cyanide Biosynthetic Genes hcnABC by the Anaerobic Regulator ANR and the Quorum-Sensing Regulators LasR and RhlR in Pseudomonas aeruginosa Journal of Bacteriology 182(24): 6940–6949
4.Kendall M. Gray et al. (1994) Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pseudomonas aeruginosa. Journal of Bacteriology 176(10): 3076–3080