Part:BBa_K395162

CmR activated by LuxR and 3OC6HSL(AHL of Lux family)

Fig.1 Growth curve of the cell introduced PlacI^q-CmR (BBa_K395165,blue curves), Plux-CmR (BBa_K395162,Red curves), or promoterless-CmR (BBa_K395160,green curves) with or without AHL (3OC6HSL).
This work is done by Yusuke Kaneta.

This device has chloramphenicol resistance (CmR) gene (BBa_K395162) downstream of lux promoter. We found that the device is activated by LuxR/3OC6HSL complex and that the cell introduced the part was able to survive even in high chloramphenicol concentration (750 ug/ml) in the presence of 3OC6HSL.(100nM)
For more information, see [http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/Cm_assay our work in Tokyo_Tech 2010 wiki]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]