Part:BBa_K1510009:Experience

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Applications of BBa_K1510009

Using yebF at the N-terminal of desired sequence as recombinant protein would help exporting it extracellularly.　Especially useful when common experimental strains, such as K-12 are used, since they don't naturally secrete protein outside.

User Reviews

UNIQf58bd13f2bda0d1b-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. This is our result under Multimode microplate reader: File:File:NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg UNIQf58bd13f2bda0d1b-partinfo-00000001-QINU