Part:BBa_K1510009:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1510009

Using yebF at the N-terminal of desired sequence as recombinant protein would help exporting it extracellularly.　Especially useful when common experimental strains, such as K-12 are used, since they don't naturally secrete protein outside.

User Reviews

UNIQ5fdf02684229cc5c-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. This is our result under Multimode microplate reader: UNIQ5fdf02684229cc5c-partinfo-00000001-QINU