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Part:BBa_C0083:Experience
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Applications of BBa_C0083
User Reviews
UNIQcda5d83ef0e7763b-partinfo-00000000-QINU
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iGEM Dundee 2011 |
We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562017). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part. |
UNIQcda5d83ef0e7763b-partinfo-00000003-QINU
Review number not understood iGEM Nagahama_2014 |
We removed LVA tag that renders protein susceptible to the action of tail-specific proteases(BBa_K1342002). And we analysed aspartic acid synthesized by E. coliJM109/BBa_K1342001. |
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